Ịba Pf/Pv Ngwa ngwa ule Antigen (Colloidal Gold)

Ịba bụ ọrịa anwụnta na-ebute, hemolytic, ọrịa na-efe efe nke na-ebute ihe karịrị nde mmadụ 200 ma na-egbu ihe karịrị otu nde mmadụ kwa afọ.Ọ bụ ụdị Plasmodium anọ kpatara ya: P. falciparum, P. vivax, P. ovale, na P. malariae.Plasmodia ndị a niile na-ebute ma na-ebibi erythrocytes mmadụ, na-emepụta oyi, ahụ ọkụ, anaemia, na splenomegaly.P. falciparum na-ebute ọrịa dị iche iche karịa ụdị plasmodial ndị ọzọ ma na-akpata ọtụtụ ọnwụ ịba.P. falciparum na P. vivax bụ ọrịa na-efe efe na-ahụkarị, mana enwere mgbanwe dị ukwuu na mpaghara na nkesa ụdị.

Na omenala, a na-achọpụta ịba site na ngosipụta nke ntule dị na Giemsa nwere ntụpọ ọbara dị n'akụkụ, na ụdị plasmodium dị iche iche na-eji ọdịdị ha dị na erythrocytes nwere ọrịa.Usoro a nwere ike ịchọta nyocha ziri ezi na nke a pụrụ ịdabere na ya, ma ọ bụ naanị mgbe ndị ọkachamara na-ahụ maka microscopists na-eji ụkpụrụ akọwapụtara, nke na-enye nnukwu ihe mgbochi maka mpaghara ụwa na ndị dara ogbenye.

Emepụtara ule ngwa ngwa ịba Pf/Pv Ag maka idozi ihe mgbochi ndị a.Ọ na-eji ọgwụ nje eme ihe kpọmkwem na P. falciparum Histidine Rich Protein-II (pHRP-II) na P. vivax Lactate Dehydrogenase (Pv-LDH) iji chọpụta n'otu oge ma chọpụta ọdịiche nke ọrịa na P. falciparum na P. vivax.Enwere ike ime ule a site n'aka ndị ọrụ na-azụghị ọzụzụ ma ọ bụ ndị nwere obere nkà, na-enweghị ngwa ụlọ nyocha

Nnwale ọsọ ọsọ nke ịba Pf/Pv Ag bụ immunoassay chromatographic n'akụkụ.Ihe nyocha nke warara nwere: 1) mpe mpe akwa conjugate na agba burgundy nwere òké mgbochi Pv-LDH mgbochi jikọtara ya na colloid gold (Pv-LDH-gold conjugates) na òké mgbochi pHRP-II mgbochi conjugated na colloid gold (pHRP-II-gold conjugates), 2 na test bandeji (PHRP-II-gold conjugates), 2) na ihe mgbochi bandeji P. band (C band).Ejiri ụzọ mgbochi Pv-LDH ọzọ kpuchie ya na mgbochi mgbochi Pv-LDH ọzọ maka ịchọpụta ọrịa Pv, Pf band na-eji polyclonal anti-pHRP-II ọgwụ mgbochi maka ịchọpụta ọrịa Pf, yana C band kpuchie ya na ewu mgbochi òké IgG.

N'oge nyocha ahụ, a na-ekesa oke nha ọbara zuru oke n'ime olulu mmiri (S) nke cassette ule, a na-agbakwunye ihe nchekwa lysis na ebe nchekwa (B).Ihe nchekwa ahụ nwere ihe nchacha nke na-emebi mkpụrụ ndụ ọbara uhie ma wepụta antigens dị iche iche, nke na-akwaga site na capillary omume n'ofe warara dị na cassette.Pv-LDH ma ọ bụrụ na ihe ngosi dị na ụdị ga-ejikọta na Pv-LDH-ọlaed conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ ahụ site na mgbochi mgbochi Pv-LDH kpuchie ya, na-akpụ eriri Pv nwere agba burgundy, na-egosi nsonaazụ Pv dị mma.N'aka nke ọzọ, pHRP-II ma ọ bụrụ na ihe ngosi dị na ụdị ga-ejikọta na pHRP-II-ọlaed conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi pHRP-II nke kpuchiri ya mbụ, na-akpụ eriri Pf nwere agba burgundy, na-egosi nsonaazụ Pf dị mma.Enweghị eriri ule ọ bụla na-egosi nsonaazụ adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ewu mgbochi ewu IgG / òké IgG (mgbochi Pv-LDH na mgbochi pHRPII) - ọla edo conjugates n'agbanyeghị mmepe agba na nke ọ bụla n'ime eriri ule.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.