Ngwa ngwa ule Leishmania IgG/IgM (Colloidal Gold)

Visceral leishmaniasis, ma ọ bụ Kala-azar, bụ ọrịa na-efe efe na-agbasa site n'ọtụtụ ụdị nke L. donovani.Òtù Ahụ Ike Ụwa (WHO) na-eme atụmatụ na ọrịa a ga-emetụta ihe dị ka nde mmadụ 12 na mba 88.A na-ebufe ya na ụmụ mmadụ site n'oriri nke ájá ájá Phlebotomus, bụ́ ndị na-ebute ọrịa site n'inye anụmanụ ndị bu ọrịa ahụ nri.Ọ bụ ezie na ọ bụ ọrịa a na-ahụ na mba ndị dara ogbenye, na Ndịda Europe, ọ ghọwo ọrịa na-ebute ohere ohere na ndị ọrịa AIDS.Nchọpụta nke L. donovani organism site na ọbara, ụmị ọkpụkpụ, imeju, lymph nodes ma ọ bụ splin na-enye nkọwa doro anya nke nchoputa.Nchọpụta serological nke mgbochi L.Achọpụtara donovani IgM ka ọ bụrụ ezigbo akara maka nnukwu leishmaniasis visceral.Nnwale a na-eji n'ụlọọgwụ gụnyere ELISA, fluorescent antibody ma ọ bụ ule agglutination ozugbo 4-5.Na nso nso a, itinye n'ọrụ nke L. donovani kpọmkwem protein n'ule emewo ka nghọta na nkọwapụta nke ọma.

Nnwale Leishmania IgG/IgM Combo Rapid bụ nnwale serological dabere na protein recombinant, nke na-achọpụta ọgwụ mgbochi IgG na IgM na L. Donovani n'otu oge.Nnwale ahụ na-enye nsonaazụ a pụrụ ịdabere na ya n'ime nkeji iri na ise na-enweghị ngwa ọ bụla.

Nnwale ọsọ ọsọ nke Leishmania IgG/IgM bụ immunoassay nke chromatographic nke mpụta.Kaseti ule ahụ nwere: 1) pad conjugate nwere agba agba burgundy nwere recombinant L. donovani antigen conjugated na colloid gold (Leishmania conjugates) na oke bekee IgG-gold conjugates, 2) nitrocellulose akpụkpọ ahụ warara nke nwere ule abụọ (T1 na T2 bands). na otu njikwa njikwa (C band).A na-ekpuchi band T1 na monoclonal mgbochi mmadụ IgM maka nchọpụta mgbochi L.Donovani IgM, T2 band ka ejiri reagents kpuchie ya maka nchọpụta mgbochi L.donovani IgG, na C band ka ejiri ewu mgbochi oke bekee IgG kpuchie ya.

Mgbe a na-ekesa oke nlere ule zuru oke n'ime olulu mmiri nke cassette ahụ, ihe atụ ahụ na-akwaga site na capillary edinam gafee cassette.L. donovani IgM ma ọ bụrụ na ọ dị na ihe nlere anya ga-ejikọta na njikọ Leishmania.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi IgM mgbochi mmadụ nke kpuchiri ya, na-eme otu band T1 nwere agba burgundy, na-egosi nsonaazụ nyocha L. donovani IgM dị mma.L. donovani IgG ma ọ bụrụ na ọ dị na ihe nlere ahụ ga-ejikọta na njikọ Leishmania.A na-ejidekwa immunocomplex ahụ site na reagents ndị etinyere mbụ mkpuchi na akpụkpọ ahụ, na-etolite band T2 nwere agba burgundy, na-egosi nsonaazụ nyocha L. donovani IgG.

Enweghị ụdọ T ọ bụla (T1 na T2) na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex ewu mgbochi oke bekee IgG/oke bekee IgG-gold conjugate n'agbanyeghị mmepe agba na ụdọ T ọ bụla.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.