Mycoplasma Pneumoniae IgG/IgM Ngwa ngwa ule ngwa ngwa (Colloidal Gold)

M.pneumoniae nwere ike ịkpata ọtụtụ mgbaàmà dị ka oyi baa n'isi, tracheobronchitis, na ọrịa akụkụ okuku ume elu.Tracheobronchitis na-adịkarị na ụmụaka nwere usoro ahụ ji alụso ọrịa ọgụ belatara, ihe ruru 18% nke ụmụaka butere ọrịa chọrọ ụlọ ọgwụ.N'ụlọ ọgwụ, M. pneumoniae enweghị ike ịdị iche na oyi baa nke nje bacteria ma ọ bụ nje ndị ọzọ na-akpata. Nchọpụta kpọmkwem dị mkpa n'ihi na ọgwụgwọ nke ọrịa M. pneumoniae na ọgwụ nje β-lactam adịghị arụ ọrụ ebe ọgwụgwọ na macrolides ma ọ bụ tetracyclines nwere ike ibelata ogologo oge nke ọrịa ahụ.

Ịrapara M. pneumoniae na epithelium iku ume bụ nzọụkwụ mbụ na usoro ọrịa.Usoro mgbakwunye a bụ ihe mgbagwoju anya nke chọrọ ọtụtụ protein adhesin, dị ka P1, P30, na P116.Ezi ọnọdụ nke ọrịa M. pneumoniae metụtara adịghị anya ka ọ na-esiri ike ịchọpụta na mmalite nke ọrịa.

Mycoplasma Pneumoniae IgG/IgM Ngwa ngwa ngwa ngwa dabere na ụkpụrụ nke qualitative immunochromatographic assay maka mkpebi nke Mycoplasma Pneumoniae IgG/IgM antibody n'ime mmadụ serum, plasma ma ọ bụ dum ọbara.StripA mejupụtara: 1) a burgundy acha conjugate conjugate conjugate conjugate conjugate nke MP2 nwere antigen. eriri akpụkpọ anụ nitrocellulose nwere eriri ule (T band) na otu njikwa (C band).Ejiri mgbochi mgbochi IgG nke òké kpuchie T band, ma ekpuchikwa band C na mgbochi ewu mgbochi òké IgG.Strip B nwere: 1) pad conjugate nwere agba agba burgundy nwere MP antigen jikọtara ya na ọla edo colloid (MP Antigen conjugates), 2) eriri akpụkpọ anụ nitrocellulose nwere eriri ule (T band) na njikwa njikwa (C band).Ejiri mgbochi mgbochi IgM nke òké kpuchie T band, ma ekpuchikwa band C na mgbochi ewu mgbochi òké IgG.

Strip A: Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette ule, ihe nlere ahụ na-akwaga site na capillary n'ofe cassette. MP IgG mgbochi ọ bụrụ na ọ dị na ụdịdị ahụ ga-ejikọta MP Antigen conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi mgbochi mmadụ IgG nke Mouse kpuchie ya, na-akpụ otu agba T nwere agba burgundy, na-egosi nsonaazụ MP IgG dị mma.Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ewu mgbochi mgbochi oke IgG/mouse IgG-gold conjugate n'agbanyeghị ọnụnọ T band nwere agba.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.

Strip B: Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette ule, ihe nlere ahụ na-akwaga site na capillary n'ofe cassette. MP IgM mgbochi ọ bụrụ na ọ dị n'ụdị ahụ ga-ejikọta MP Antigen conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ ahụ site na mgbochi mgbochi IgM nke Mouse kpuchie tupu ya, na-akpụ otu agba T nwere agba burgundy, na-egosi nsonaazụ MP IgM dị mma.Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ewu mgbochi mgbochi oke IgG/mouse IgG-gold conjugate n'agbanyeghị ọnụnọ T band nwere agba.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.