Ngwa ịba Pf/Pan Antigen Rapid Test (Colloidal Gold)

Ịba bụ ọrịa anwụnta na-ebute, hemolytic, ọrịa na-efe efe nke na-ebute ihe karịrị nde mmadụ 200 ma na-egbu ihe karịrị otu nde mmadụ kwa afọ.Ọ bụ ụdị Plasmodium anọ kpatara ya: P. falciparum, P. vivax, P. ovale, na P. malariae.Plasmodia ndị a niile na-ebute ma na-ebibi erythrocytes mmadụ, na-emepụta oyi, ahụ ọkụ, anaemia, na splenomegaly.P. falciparum na-ebute ọrịa dị iche iche karịa ụdị plasmodial ndị ọzọ ma na-akpata ọtụtụ ọnwụ ịba.P. falciparum na P. vivax bụ ọrịa na-efe efe na-ahụkarị, mana enwere mgbanwe dị ukwuu na mpaghara na nkesa ụdị.

Na omenala, a na-achọpụta ịba site na ngosipụta nke ntule dị na Giemsa nwere ntụpọ ọbara dị n'akụkụ, na ụdị plasmodium dị iche iche na-eji ọdịdị ha dị na erythrocytes nwere ọrịa1.Usoro a nwere ike ịchọpụta nyocha ziri ezi na nke a pụrụ ịdabere na ya, ma ọ bụ naanị mgbe ndị ọkachamara na-ahụ maka microscopists na-eji protocols2 akọwapụtara, nke na-enye nnukwu ihe mgbochi maka mpaghara ụwa na ndị dara ogbenye.

Emebere ngwa ịba Pf / Pan Antigen Rapid Test Kit maka idozi ihe mgbochi ndị a.Nnwale ahụ na-eji otu ụzọ monoclonal na polyclonal antibodies na P. falciparum kpọmkwem protein, Histidine Repeat Protein II (pHRP-II), na otu ụzọ mgbochi monoclonal na plasmodium Lactate Dehydrogenase (pLDH), protein nke ụdị anọ nke plasmodium na-emepụta, si otú a na-eme ka P. .Enwere ike ịrụ ya site n'aka ndị ọrụ na-agụghị akwụkwọ ma ọ bụ ndị nwere obere nkà, na-enweghị akụrụngwa ụlọ nyocha.

Ngwa nyocha ngwa ngwa nke Pf/ Pan ịba bụ immunoassay chromatographic nke mpụta.Ihe mejupụtara ihe nlere ule ahụ nwere: 1) mpe mpe akwa conjugate nwere agba burgundy nwere mgbochi pHRP-II nke ejikọrọ na ọla edo colloid (pHRP II-gold conjugates) na mgbochi pLDH òké jikọtara ya na ọla edo colloid (pLDH-gold conjugates),

2) eriri akpụkpọ anụ nitrocellulose nwere eriri ule abụọ (Pan na Pv band) na otu njikwa (C band).A na-ekpuchi pan band na monoclonal anti-pLDH antibody nke enwere ike ịchọpụta ọrịa nke ọ bụla n'ime ụdị anọ nke plasmodia, Pf band bụ nke ejiri polyclonal anti-pHRP-II antibodies kpuchie ya maka nchọpụta nke ọrịa Pf, na C band na-ekpuchi ya na ewu mgbochi òké IgG.

N'oge nyocha ahụ, a na-ekesa oke nha ọbara zuru oke n'ime olulu mmiri (S) nke cassette ule, a na-agbakwunye ihe nchekwa lysis na ebe nchekwa (B).Ihe nchekwa ahụ nwere ihe nchacha nke na-emebi mkpụrụ ndụ ọbara uhie ma wepụta plasmodium antigens dị iche iche, nke na-akwaga site na capillary omume n'ofe warara dị na cassette.pHRP-II ma ọ bụrụ na ihe ngosi dị na ụdị ga-ejikọta na pHRP II-ọlaedo conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi pHRPII kpuchiri mbụ, na-akpụ eriri Pf nwere agba burgundy, na-egosi nsonaazụ Pf dị mma.pLDH ma ọ bụrụ na onyinye dị n'ụdị ga-ejikọta na pLDH conjugates ọla edo.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi pLDH mkpuchi nke mbụ, na-akpụ eriri Pan nwere agba burgundy, na-egosi nsonaazụ plasmodium ziri ezi.Na enweghị Pan band, enwere ike ịkwado nsonaazụ ule dị mma maka plasmodia atọ ndị ọzọ.

Enweghị eriri ule ọ bụla (Pan na Pf) na-egosi nsonaazụ adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta otu agba agba agba burgundy nke immunocomplex nke ewu mgbochi òké IgG / òké IgG (pHRP-II na pLDH-gold conjugates) n'agbanyeghị mmepe agba na ụcha ule ọ bụla.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.