Ngwa nyocha ngwa ngwa ịba Pf Antigen (Colloidal Gold)

Ịba bụ ọrịa anwụnta na-ebute, hemolytic, ọrịa na-efe efe nke na-ebute ihe karịrị nde mmadụ 200 ma na-egbu ihe karịrị otu nde mmadụ kwa afọ.Ọ bụ ụdị Plasmodium anọ kpatara ya: P. falciparum, P. vivax, P. ovale, na P. malariae.Plasmodia ndị a niile na-ebute ma na-ebibi erythrocytes mmadụ, na-emepụta oyi, ahụ ọkụ, anaemia, na splenomegaly.P. falciparum na-ebute ọrịa dị iche iche karịa ụdị plasmodial ndị ọzọ na-akpata ọtụtụ ọnwụ ịba, ọ bụkwa otu n'ime ọrịa ịba abụọ a na-ahụkarị.

Na omenala, a na-achọpụta ịba site na ngosipụta nke ntule dị na Giemsa nwere ntụpọ ọbara dị n'akụkụ, na ụdị plasmodium dị iche iche na-eji ọdịdị ha dị na erythrocytes nwere ọrịa.Usoro a nwere ike ịchọta nyocha ziri ezi na nke a pụrụ ịdabere na ya, ma ọ bụ naanị mgbe ndị ọkachamara na-ahụ maka microscopists na-eji ụkpụrụ akọwapụtara, nke na-enye nnukwu ihe mgbochi maka mpaghara ụwa na ndị dara ogbenye.

Emepụtara Pf Ag Rapid Test maka idozi ihe mgbochi ndị a.Ọ na-achọpụta Pf kpọmkwem antigen pHRP-II n'ime ọbara mmadụ.Enwere ike ịrụ ya site n'aka ndị ọrụ na-agụghị akwụkwọ ma ọ bụ ndị nwere obere nkà, na-enweghị akụrụngwa ụlọ nyocha.

Nnwale Pf Ag Rapid bụ immunoassay chromatographic nke mpụta.Ihe ndị mejupụtara ule nnwale ahụ nwere: 1) pad conjugate nwere agba agba burgundy nwere mgbochi pHRP-II monoclonal jikọtara ya na ọla edo colloid (pHRP II-gold conjugates), 2) eriri akpụkpọ anụ nitrocellulose nwere eriri nnwale (Pf) na igwe njikwa (C band).A na-eji ọgwụ mgbochi pHRP-II polyclonal kpuchie Pf band, ma kpuchie band C na ewu mgbochi òké IgG.

N'oge nyocha ahụ, a na-ekesa oke nha ọbara zuru oke n'ime olulu mmiri (S) nke cassette ule, a na-agbakwunye ihe nchekwa lysis na ebe nchekwa (B).Ihe nchekwa ahụ nwere ihe nchacha nke na-emebi mkpụrụ ndụ ọbara uhie wee wepụta antigen pHRPII, nke na-akwaga site na capillary omume n'ofe warara ejidere na cassette.pHRP-II ma ọ bụrụ na ihe ngosi dị na ụdị ga-ejikọta na pHRP II-ọlaedo conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na ọgwụ mgbochi polyclonal antipHRP II nke kpuchiri ya mbụ, na-akpụ eriri Pf nwere agba burgundy, na-egosi nsonaazụ Pf dị mma.

Enweghị Pf band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta otu agba agba agba burgundy nke immunocomplex nke ewu mgbochi òké IgG / òké IgG (pHRP II-gold conjugates) n'agbanyeghị mmepe agba na otu Pf ọ bụla.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.