Ngwa nyocha ngwa ngwa nke Cholera Ag (Colloidal Gold)

Ọgbụgbọ ọgbụgbọ bụ nnukwu ọrịa na-efe efe nke a na-eji oke mfu nke mmiri ahụ na electrolytes site na afọ ọsịsa siri ike.Achọpụtala ihe na-ahụ maka etiological nke ọgbụgbọ ọgbụgbọ dị ka Vibrio cholerea (V. Cholerae), nje bacteria na-adịghị mma nke gram, nke a na-ebutekarị mmadụ site na mmiri na nri emeru.

A na-ekewa ụdị V. Cholerae n'ime ọtụtụ serogroups na ndabere nke O antigens.Otu obere O1 na O139 nwere mmasị pụrụ iche n'ihi na ha abụọ nwere ike ibute ọrịa na-efe efe na ọrịa ọgbụgbọ ọgbụgbọ.Ọ dị oke mkpa ịchọpụta ngwa ngwa o kwere omume ọnụnọ V. cholerae O1 na O139 n'ụdị ụlọ ọgwụ, mmiri na nri ka ndị ọrụ ahụike ọha wee nwee ike were nleba anya kwesịrị ekwesị na mgbochi dị mma.

Enwere ike iji ule Cholera Ag ngwa ngwa n'ọhịa site n'aka ndị ọrụ na-azụghị akwụkwọ ma ọ bụ ndị nwere obere nka na nsonaazụ ya dị n'ihe na-erughị nkeji iri, na-enweghị ngwa ụlọ nyocha dị egwu.

Cholera Ag Rapid Test bụ n'akụkụ mpụta chromatographic immunoassay.Kaseti ule ahụ nwere: 1) pad conjugate nwere agba agba burgundy nwere mgbochi-V monoclonal.Cholera O1 na O139 mgbochi ọrịa jikọtara ya na colloid gold (O1/O139-antibody conjugates) na oke bekee IgG-gold conjugates, 2) a nitrocellulose akpụkpọ eriri nwere ule abụọ band (1 na 139 bands) na a akara band (C band).Ekpuchiri band 1 ahụ na monoclonal anti-V.Cholera O1 mgbochi.Ejiri 139 gbachie ya na monoclonal anti-V.Cholera O139 mgbochi.Ejikwa mgbochi ewu IgG mgbochi ewu kpuchie bandeji C.

Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette, ihe nlere ahụ na-akwaga site na capillary omume gafee cassette ahụ.V. Cholera O1/O139 antigen ma ọ bụrụ na ọ dị na ụdị a ga-ejikọta na O1/O139-antibody gold conjugate kwekọrọ.A na-ejidekwa immunocomplex a na akpụkpọ ahụ site na mgbochi V nke ekpuchiburu ya.Cholera O1/O139 mgbochi ọrịa, na-akpụ eriri ule agba agba burgundy, na-egosi nsonaazụ ule Cholera O1/O139 dị mma.Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.

Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex ewu anti-mouse IgG/ òké IgG-gold conjugate n'agbanyeghị mmepe agba na band ule.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.