Ngwa nyocha ngwa ngwa nke Chikungunya IgG/IgM+NS1 (Colloidal Gold)

Chikungunya bụ ọrịa nje na-adịghị ahụkebe nke anwụnta Aedes aegypti butere na-ebute.Ihe e ji mara ya bụ ọkụ ọkụ, ahụ ọkụ, na mgbu nkwonkwo siri ike (arthralgias) nke na-adịrukarị ụbọchị atọ ruo asaa.Aha a sitere na okwu Makonde nke pụtara "nke na-ehulata" n'izo aka n'ọnọdụ gbadara agbagọ nke mepụtara n'ihi mgbaàmà ogbu na nkwonkwo nke ọrịa ahụ.Ọ na-eme n'oge mmiri ozuzo

na mpaghara okpomọkụ nke ụwa, nke kachasị na Africa, South-East Asia, South India na Pakistan.Ihe mgbaàmà na-abụkarị nke a na-apụghị ịmata nke ọma n'ụlọ ọgwụ ndị a na-ahụ na ahụ ọkụ dengue.N'ezie, a kọwo ọrịa dengue na chikungunya ugboro abụọ n'India.N'adịghị ka dengue, ngosipụta hemorrhagic na-adịkarị ụkọ na ọtụtụ mgbe ọrịa ahụ bụ ọrịa na-egbochi onwe ya.Ya mere ọ dị ezigbo mkpa

iji chọpụta ọdịiche dengue na ọrịa CHIK.Achọpụtara CHIK dabere na nyocha serological na ikewapụ nje na ụmụ oke ma ọ bụ omenala anụ ahụ.IgM immunoassay bụ usoro nyocha ụlọ nyocha kachasị dị irè.Nnwale ngwa ngwa nke Chikungunya IgG/IgM na-eji antigens recombinant ewepụtara na protein nhazi ya, ọ na-achọpụta IgG/IgM mgbochi CHIK na serum ma ọ bụ plasma onye ọrịa n'ime nkeji iri abụọ.Enwere ike ime ule ahụ site na ndị na-azụghị akwụkwọ ma ọ bụ

ndị ọrụ nwere obere nka, na-enweghị akụrụngwa ụlọ nyocha dị egwu.

Nnwale ngwa ngwa nke Chikungunya IgG/IgM+NS1 bụ immunoassay chromatographic nke mpụta.Kaseti ule nwere IgG/IgM Strip na NS1 strip.

IgG/IgM strip: 1) pad conjugate na-acha uhie uhie nwere Chikungunya recombinant envelopu antigens jikọtara ya na colloid gold (dengue conjugates) na oke bekee IgG-gold conjugates,2) nitrocellulose akpụkpọ anụ eriri nwere ule abụọ (G na M bands) na a njikwa band (C band).A na-etinye ihe mgbochi G band maka nchọpụta IgG anti-Chikungunya nje, M band a na-ekpuchikwa ọgwụ mgbochi maka nchọpụta IgM anti-Chikungunya nje, na C band bụ tupu mkpuchi ewu mgbochi oke bekee IgG.

NS1 strip:1) pad conjugate na-acha agba burgundy nwere òké mgbochi Chikungunya NS1 antigen conjugated na colloid gold (Chikungunya Ab conjugates), 2) nitrocellulose membrane strip nwere a test band (T band) na njikwa band (C band).Ejikwa antigen-Chikungunya NS1 oke bekee kpuchie T band, ma were mgbochi ewu na-egbochi òké IgG kpuchie otu C.

IgG/IgM strip: Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette, ihe nlere ahụ na-akwaga site na capillary omume gafee cassette ahụ.Nje Virus IgG na-egbochi Chikungunya ma ọ bụrụ na ọ dị n'ụdị a ga-ejikọta ya na njikọ Chikungunya.A na-ejidekwa immunocomplex ahụ site na reagent mkpuchi mkpuchi na band G, na-akpụ otu agba agba burgundy G, na-egosi nsonaazụ ule ọma nke Chikungunya IgG ma na-atụ aro ọrịa na-adịbeghị anya ma ọ bụ megharịa.Nje virus nke IgM na-egbochi Chikungunya, ọ bụrụ na ọ dị na ụdị ihe atụ ahụ, ga-ejikọta na njikọ nke Chikungunya.A na-ejidekwa immunocomplex ahụ site na reagent kpuchiri ya na band M, na-akpụ eriri M nwere agba burgundy, nke na-egosi nsonaazụ nyocha nke ọma nke Chikungunya IgM ma na-atụ aro ka ọ bute ọrịa ọhụrụ.Enweghị eriri ule ọ bụla (G na M) na-egosi nsonaazụ na-adịghị mma. Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ewu mgbochi oke bekee IgG/

oke bekee IgG-ọlaedo conjugate n'agbanyeghị mmepe agba na nke ọ bụla n'ime ìgwè T.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.

NS1 Strip: Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nke cassette ahụ, ihe nlere ahụ na-akwaga site na capillary omume gafee cassette ule.Chikungunya NS1 Ag ọ bụrụ na ọ dị na ụdị ihe atụ ga-ejikọta na njikọ Chikungunya Ab.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi bekee mgbochi NS1, na-akpụ otu agba T nwere agba burgundy, na-egosi nsonaazụ Chikungunya Ag dị mma.Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosi eriri agba agba burgundy nke immunocomplex nke ewu mgbochi mgbochi oke IgG/mouse IgG-gold conjugate n'agbanyeghị ọnụnọ T band nwere agba.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.