Ngwa ngwa ule Chikungunya IgG/IgM (Colloidal Gold)

Chikungunya bụ ọrịa nje na-adịghị ahụkebe nke anwụnta Aedes aegypti butere na-ebute.Ihe e ji mara ya bụ ọkụ ọkụ, ahụ ọkụ, na mgbu nkwonkwo siri ike (arthralgias) nke na-adịrukarị ụbọchị atọ ruo asaa.Aha a sitere na okwu Makonde nke pụtara "nke na-ehulata" n'izo aka n'ọnọdụ gbadara agbagọ nke mepụtara n'ihi mgbaàmà ogbu na nkwonkwo nke ọrịa ahụ.Ọ na-eme n'oge mmiri ozuzo na mpaghara okpomọkụ nke ụwa, karịsịa na Africa, South-East Asia, South India na Pakistan.Ihe mgbaàmà na-abụkarị nke a na-apụghị ịmata nke ọma n'ụlọ ọgwụ ndị a na-ahụ na ahụ ọkụ dengue.N'ezie, a kọwo ọrịa dengue na chikungunya ugboro abụọ n'India.N'adịghị ka dengue, ngosipụta hemorrhagic na-adịkarị ụkọ na ọtụtụ mgbe ọrịa ahụ bụ ọrịa na-egbochi onwe ya.Ya mere, ọ dị ezigbo mkpa ịmata ọdịiche nke dengue na ọrịa CHIK.Achọpụtara CHIK dabere na nyocha serological na ikewapụ nje na ụmụ oke ma ọ bụ omenala anụ ahụ.IgM immunoassay bụ usoro nyocha ụlọ nyocha kachasị dị irè.Nnwale ngwa ngwa nke Chikungunya IgG/IgM na-eji antigens recombinant ewepụtara na protein nhazi ya, ọ na-achọpụta IgG/IgM mgbochi CHIK na serum ma ọ bụ plasma onye ọrịa n'ime nkeji iri abụọ.Enwere ike ime ule ahụ site na ndị na-azụghị akwụkwọ ma ọ bụ

ndị ọrụ nwere obere nka, na-enweghị akụrụngwa ụlọ nyocha dị egwu.

Nnwale ngwa ngwa nke Chikungunya IgG/IgM bụ immunoassay chromatographic n'akụkụ.Kaseti ule ahụ nwere: 1) pad conjugate na-acha agba burgundy nwere Chikungunya recombinant envelopu antigens conjugated na colloid gold (dengue conjugates) na oke bekee IgG-gold conjugates,2) nitrocellulose akpụkpọ ahụ strip nwere ule abụọ ule band (G na M bands) na otu akara njikwa).A na-etinye ihe mgbochi G band maka nchọpụta IgG anti-Chikungunya nje, M band a na-ekpuchikwa ọgwụ mgbochi maka nchọpụta IgM anti-Chikungunya nje, na C band bụ tupu mkpuchi ewu mgbochi oke bekee IgG.

Mgbe a na-ekesa oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette ahụ, ihe nlere ahụ na-akwaga site na capillary edinam gafee cassette.Nje Virus IgG na-egbochi Chikungunya ma ọ bụrụ na ọ dị n'ụdị a ga-ejikọta ya na njikọ Chikungunya.Enweghi ike iji reagents nke ọnụọgụ batch dị iche iche na-agbanwe agbanwe. A na-ejidekwa immunocomplex site na reagent mkpuchi na band G, na-akpụ otu agba agba burgundy G, na-egosi nje virus Chikungunya IgG nti ule na-atụ aro na nso nso a ma ọ bụ ugboro ugboro ọrịa.Nje Virus IgM na-egbochi Chikungunya, ọ bụrụ na ọ dị na ụdị ihe atụ a, ga-ejikọta ya na njikọ Chikungunya.A na-ejidekwa immunocomplex ahụ site na reagent kpuchiri ya na band M, na-akpụ eriri M nwere agba burgundy, nke na-egosi nsonaazụ nyocha nke ọma nke Chikungunya IgM ma na-atụ aro ọrịa ọhụrụ.Enweghị eriri ule ọ bụla (G na M) na-egosi nsonaazụ na-adịghị mma. Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosi eriri agba agba burgundy nke immunocomplex ewu anti rabbit IgG / rabbit IgG-gold conjugate n'agbanyeghị mmepe agba na nke ọ bụla n'ime ìgwè T.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.