Nkọwa zuru ezu
Tụlee ihe ọ bụla sitere na mmadụ ka ọ na-efe efe ma jikwaa ya site na iji usoro biosafety ọkọlọtọ.
Plasma
1. Chịkọta ụdị ọbara n'ime lavender, acha anụnụ anụnụ ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ top collection tube (nwere EDTA, citrate ma ọ bụ heparin, karị na Vacutainer®) site na veinpuncture.
2. Kewaa plasma site na centrifugation.
3.Crefully ịdọrọ plasma n'ime ọhụrụ tupu akara tube.
Ọbara
1. Chịkọta ụdị ọbara n'ime tube mkpokọta na-acha uhie uhie (nke enweghị ọgwụ mgbochi ọbara na Vacutainer®) site na veinpuncture.
2.Kwe ka ọbara kụọ.
3. Kewaa serum site centrifugation.
4.Crefully ịdọrọ serum n'ime a ọhụrụ akpa akara tube.
5.Test ụdị ngwa ngwa o kwere omume mgbe ịnakọta.Na-echekwa ụdịdị na 2°C ruo 8°C ma ọ bụrụ na anwaleghị ya ozugbo.
6.Chekwa ụdịdị na 2°C ruo 8°C ruo ụbọchị 5.A ga-ekporịrị ụdịdị ahụ na -20 Celsius maka nchekwa ogologo oge
Ọbara
Enwere ike nweta ntụpọ nke ọbara dum site na nkuchi aka n'ọnụ mkpịsị aka ma ọ bụ veinpuncture.Ejila ọbara hemolaized ọ bụla maka nnwale.Ekwesịrị ịchekwa ụdị ọbara niile na friji (2°C-8°C) ma ọ bụrụ na anwaleghị ya ozugbo.A ga-anwale ihe nlere ahụ n'ime awa 24 nke nchịkọta. Zere ọtụtụ okirikiri oyi-ajụ oyi.Tupu anwale, weta ụdị oyi kpọnwụrụ n'ime ụlọ ahụ nwayọ wee gwakọta nwayọ.A ga-akọwapụta ihe nlere nwere akụkụ ahụ a na-ahụ anya site na centrifugation tupu nnwale.
Usoro ASsay
Kwụpụ 1: Weta ihe nlere ahụ wee nwalee ihe mejupụtara ya n'ime ụlọ ma ọ bụrụ na etinyere ya na friji ma ọ bụ friji.Ozugbo agbazere, gwakọta ihe nlele ahụ nke ọma tupu ị nyocha.
Kwụpụ 2: Mgbe ị dị njikere ịnwale, mepee obere akpa na notch wee wepụ ngwaọrụ.Tinye ngwaọrụ nnwale n'elu ala dị ọcha ma dịkwa larịị.
Kwụpụ 3: Jide n'aka na ị ga-eji akara njirimara kanye ngwaọrụ ahụ.
Kwụpụ 4: Maka ule ọbara dum - Tinye 1 dobe ọbara dum (ihe dịka 30-35 µL) n'ime nlele nke ọma.- Mgbe ahụ tinye 2 tụlee (ihe dị ka 60-70 µL) nke Sample Diluent ozugbo.