NKỌWA NA NKWAWA NWA Nnwale
Ọrịa afọ ọsịsa bụ otu n'ime ụkpụrụ na-ebute ọrịa nwata na ọnwụ n'ụwa niile, na-ebute ọnwụ nde 2.5 kwa afọ.Ọrịa Rotavirus bụ ihe na-ebute ọrịa afọ ọsịsa siri ike na ụmụ ọhụrụ na ụmụaka na-erubeghị afọ ise, na-akpata 40%-60% nke nnukwu gastroenteritis na-akpata ma na-eme atụmatụ ọnwụ ụmụaka 500,000 kwa afọ.Ka ọ na-erule afọ ise, ihe fọrọ nke nta ka ọ bụrụ ụmụaka ọ bụla nọ n'ụwa ebutela ọrịa rotavirus opekata mpe otu ugboro.Site na ọrịa ndị na-esote, a na-ebute nzaghachi mgbochi heterotypic sara mbara;ya mere, ndị okenye anaghị emetụta ya.
Ka ọ dị ugbu a, otu asaa nke rotaviruses (otu AG) kewapụrụ na
ji mara.Otu rotavirus rotavirus na-ebutekarị ọrịa Rotavirus n'ime mmadụ karịa 90%.A na-ebunye Rotavirus n'ụzọ bụ isi site na ụzọ fecaloral, ozugbo site na mmadụ gaa na onye.Titers nje na stool na-eru oke obere obere oge ka ọrịa malitere, wee daa.Oge nnabata nke ọrịa rotavirus na-abụkarị otu ụbọchị ruo ụbọchị atọ ma gastroenteritis na-esote ya na nkezi oge nke ụbọchị atọ ruo ụbọchị asaa.Mgbaàmà nke ọrịa ahụ sitere na afọ ọsịsa dị nro, afọ ọsịsa na oke afọ ọsịsa nwere ahụ ọkụ na vomiting.
Enwere ike ịchọpụta ọrịa rotavirus na-esochi nchoputa nke gastroenteritis dị ka ihe na-ebute afọ ọsịsa siri ike na ụmụaka.N'oge na-adịbeghị anya, nchoputa a kapịrị ọnụ nke ọrịa na rotavirus apụtala site na nchọpụta nje antigen na stool site na usoro immunoassay dị ka latex agglutination assay, EIA, na mpụta chromatographic immunoassay.
Nnwale Rotavirus Ag Rapid bụ mpụta chromatographic immunoassay nke na-eji otu ụzọ mgbochi mgbochi iji chọpụta nke ọma na rotavirus antigen n'ime ụdị fecal.Enwere ike ịme ule ahụ na-enweghị akụrụngwa ụlọ nyocha dị egwu, nsonaazụ ya dị n'ime nkeji iri na ise.
Ụkpụrụ
Nnwale Rotavirus Ag ngwa ngwa bụ immunoassay chromatographic nke mpụta.Ihe nlere ule ahụ nwere: 1) pad conjugate na-acha odo odo nwere monoclonal anti-rotavirus antibody jikọtara ya na colloidal gold (anti-rotavirus conjugates) na ihe mgbochi mgbochi conjugated na colloidal gold, 2) nitrocellulose akpụkpọ ahụ nke nwere eriri ule (T). ahịrị) na akara akara (akara C).A na-eji ọgwụ mgbochi rotavirus monoclonal ọzọ kpuchie T ahịrị, ma jiri eriri akara mgbochi kpuchie eriri C.
Mgbe etinyere oke nlere ewepụtara n'ime olulu mmiri nlele nke cassette ahụ, ihe nlere ahụ na-akwaga site na capillary omume gafee cassette ahụ.Rotavirus Ag, ọ bụrụ na ọ dị na ụdị, ga-ejikọta na conjugates mgbochi rotavirus.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi rotavirus kpuchiri mbụ nke na-emepụta akara T nke nwere agba burgundy, na-egosi nsonaazụ nyocha rotavirus. na-egosi nsonaazụ ọjọọ rotavirus.Nnwale ahụ nwere njikwa ime (C line), nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ọgwụ mgbochi njikwa, n'agbanyeghị mmepe agba na ahịrị T.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.
