NKỌWA NA NKWAWA NWA Nnwale
Ọrịa lymphatic filariasis nke a maara dị ka Elephantiasis, bụ nke W. bancrofti na B. malayi kpatara, na-emetụta ihe dị ka nde mmadụ 120 karịa mba 80.A na-ebute ọrịa a na ụmụ mmadụ site n'agbụ anwụnta ndị bu ọrịa nke microflariae amịpụtara site n'ahụ mmadụ nwere ọrịa na-etolite ghọọ larvae nke atọ.N'ozuzu, ikpughe ugboro ugboro na ogologo ogologo oge na larvae bu ọrịa a chọrọ iji guzobe ọrịa mmadụ.
Nchọpụta parasitologic pụtara ìhè bụ ngosipụta nke microflariae na nlele ọbara.Agbanyeghị, amachibidoro ọkọlọtọ ọla edo a site na ihe achọrọ maka nchịkọta ọbara nke abalị na enweghị ezi uche zuru oke.Nchọpụta antigens na-ekesa dị na ahịa.Uru ya bụ oke maka W. bancrofti.Na mgbakwunye, microfilaremia na antigenemia na-etolite site na ọnwa ruo afọ mgbe ekpughere ya.
Nchọpụta ihe mgbochi na-enye ụzọ mbụ iji chọpụta ọrịa nje nje filarial.Ọnụnọ nke IgM na antigens parasite na-egosi ọrịa ugbu a, ebe IgG kwekọrọ na njedebe nke ọrịa ma ọ bụ ọrịa gara aga.Ọzọkwa, njirimara nke antigens echekwara na-enye ohere nnwale 'pan-filaria' dị.Iji protein recombinant eme ihe na-ewepụ mmeghachi omume na ndị nwere ọrịa nje ndị ọzọ.
Nnwale Filariasis Ab Rapid na-eji antigens recombinant echekwara n'otu oge chọpụta Antibody na W. bancrofti na nje nje B. malayi na-enweghị mmachi na nchịkọta ihe atụ.
Ụkpụrụ
Nnwale Filariasis Ab Rapid bụ immunoassay nke chromatographic n'akụkụ.Kaseti ule ahụ nwere: 1) pad conjugate nwere agba burgundy nwere recombinant Filariasis kpọmkwem antigen conjugated na colloid gold (Filariasis conjugates) na oke bekee IgG-gold conjugates, 2) nitrocellulose akpụkpọ ahụ warara nwere a ule band (T band) na njikwa. band (C band).A na-eji antigen Filariasis na-abụghị conjugated kpuchie T band, na C-band a na-eji ewu mgbochi oke bekee IgG kpuchie ya.
Mgbe a na-ekesa oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette ahụ, ihe nlere ahụ na-akwaga site na capillary edinam gafee cassette.AntiFilariasis Ab ma ọ bụrụ na ọ dị n'ụdị a ga-ejikọta na conjugates Filariasis.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na antigen nke ekpuchiri mbụ, na-akpụ otu agba T nke nwere agba burgundy, na-egosi nsonaazụ aFilariasis Ab.Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosi eriri agba agba burgundy nke immunocomplex ewu mgbochi oke bekee IgG/oke bekee IgG-gold conjugate n'agbanyeghị mmepe agba na band T.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.