NKỌWA NA NKWAWA NWA Nnwale
Nje virus Dengue, ezinụlọ nwere ụdị nje anọ dị iche iche (Den 1,2,3,4), bụ nje virus RNA nwere naanị otu, kpuchie, nwee ezi uche.Ọ bụ anwụnta nke ezinụlọ Stegemia na-ata ahụhụ ụbọchị, nke bụ́ isi Aedes aegypti, na Aedes albopictus na-ebufe nje a.Taa, ihe karịrị ijeri mmadụ 2.5 bi n’ebe ndị dị n’ebe okpomọkụ Eshia, Africa, Australia, na America nọ n’ihe ize ndụ nke ibute ọrịa dengue.E mere atụmatụ na ihe dị ka nde ọrịa dengue 100 nde na ọrịa 250,000 nke ahụ ọkụ dengue hemorrhagic na-eyi ndụ egwu na-eme kwa afọ n'ụwa nile.
Nchọpụta serological nke ọgwụ mgbochi IgM bụ usoro a na-ahụkarị maka nyocha nke ọrịa dengue.N'oge na-adịbeghị anya, nchọpụta nke antigens ewepụtara n'oge njegharị nje na onye ọrịa ahụ gosipụtara nsonaazụ na-ekwe nkwa.Ọ na-enyere nchoputa site na ụbọchị mbụ mgbe ahụ ọkụ malitere ruo ụbọchị 9, ozugbo usoro ọgwụgwọ nke ọrịa ahụ kwụsịrị, si otú a na-enye ohere ọgwụgwọ n'oge na-etinye ya ozugbo4-. A na-emepụta Dengue NS1 ngwa ngwa Test iji chọpụta na-ekesa dengue antigen na serum. ,plasma ma ọ bụ ọbara dum.Enwere ike ime ule a site n'aka ndị ọrụ na-agụghị akwụkwọ ma ọ bụ ndị nwere obere nkà, na-enweghị ngwa ụlọ nyocha.
Ụkpụrụ
Nnwale Dengue NS1 ngwa ngwa bụ immunoassay chromatographic nke mpụta.Kaseti ule ahụ nwere: 1) pad conjugate nwere agba burgundy nwere òké mgbochi dengue NS1 antigen conjugated na colloid gold (Dengue Ab conjugates), 2) nitrocellulose membrane strip nwere a test band (T band) na akara njikwa (C). band).Ejikwa mgbochi dengue NS1 antigen òké kpuchie ya na band C
a na-ebu ụzọ kpuchie ya na mgbochi òké IgG mgbochi ewu.Ihe mgbochi maka dengue antigen na-amata antigens sitere na serotypes anọ nke nje dengue.
Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nke cassette ahụ, ihe nlere ahụ na-akwaga site na capillary edinam gafee cassette ule.Dengue NS1 Ag ma ọ bụrụ na ọ dị na ụdị a ga-ejikọta na dengue Ab conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ ahụ site na mgbochi antiNS1 òké kpuchiri ya, na-akpụ otu agba T nwere agba burgundy, na-egosi nsonaazụ Dengue Ag dị mma.
Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosi eriri agba agba burgundy nke immunocomplex nke ewu mgbochi mgbochi oke IgG/mouse IgG-gold conjugate n'agbanyeghị ọnụnọ T band nwere agba.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.
