Ngwa ngwa ule Chlamydia Pneumoniae IgG/IgM (Colloidal Gold)

Chlamydia pneumoniae (C. pneumoniae) bụ ụdị nje bacteria na-emekarị na ihe bụ isi na-akpata oyi baa gburugburu ụwa.Ihe dị ka 50% nke ndị toro eto nwere ihe akaebe nke ọrịa gara aga site na afọ 20, na ịmaliteghachi n'oge ndụ na-adịkarị.Ọtụtụ nnyocha egosiwo njikọ kpọmkwem n'etiti ọrịa C. pneumoniae na ọrịa ndị ọzọ na-egbuke egbuke dị ka atherosclerosis, nnukwu exacerbations nke COPD, na ụkwara ume ọkụ.

Nchọpụta nke ọrịa C. pneumoniae bụ ihe ịma aka n'ihi ọdịdị ngwa ngwa nke nje na-akpata, nnukwu seroprevalence, na ohere nke ụgbọ ala asymptomatic na-agafe agafe.Ewubere diagnostic laabu ụzọ gụnyere iche nke organism na cell omenala, serological assays na PCR.Microimmunofluorescence test (MIF), bụ ugbu a "ọkọlọtọ ọla edo" maka serological nchoputa, ma assay ka enweghị standardization na-teknuzu ịma aka.Immunoassay nke mgbochi bụ ule serology a na-ejikarị eme ihe yana ọrịa chlamydial bụ isi bụ nzaghachi IgM kachasị n'ime izu 2 ruo 4 yana nzaghachi IgG na IgA na-egbu oge n'ime izu 6 ruo 8.Otú ọ dị, na nlọghachi azụ, ọkwa IgG na IgA na-ebili ngwa ngwa, mgbe mgbe n'ime izu 1-2 ebe a na-adịkarịghị achọpụta ọkwa IgM.N'ihi nke a, ọgwụ mgbochi IgA egosila na ọ bụ akara immunological nke a pụrụ ịdabere na ya nke isi, ọrịa na-adịghị ala ala na nke na-emekarị mgbe ọ na-ejikọta ya na nchọpụta IgM.

Chlamydia pneumoniae IgG/IgM Ngwa ngwa ngwa ngwa ngwa dabere na ụkpụrụ nke qualitative immunochromatographic assay maka mkpebi nke Chlamydia pneumoniae IgG/IgM antibody n'ime mmadụ serum, plasma ma ọ bụ dum ọbara.StripA mejupụtara: 1) a burgundy acha ọbara ọbara nwere c. pneumoniae Antigen conjugates), 2) eriri akpụkpọ anụ nitrocellulose nwere eriri ule (T band) na njikwa njikwa (C band).Ejiri mgbochi mgbochi IgG nke òké kpuchie T band, makwa otu C na mgbochi ewu mgbochi òké IgG.Strip B nwere: 1) pad conjugate nwere agba agba burgundy nwere C. pneumoniae antigen jikọtara ya na colloid gold (C. pneumoniae Antigen conjugates), 2) a

nitrocellulose membrane strip nwere eriri ule (T band) na otu njikwa (C band).Ejiri mgbochi mgbochi IgM nke òké kpuchie T band, ma echiekwa band C na mgbochi ewu mgbochi òké IgG.

Strip A: Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette, ihe nlere ahụ na-akwaga site na capillary action gafee cassette.C.pneumoniae IgG mgbochi ọ bụrụ na ọ dị n'ime ihe nlele ahụ ga-ejikọta C. pneumoniae Antigen conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi mgbochi mmadụ IgG nke Mouse kpuchie ya, na-eme otu agba T nwere agba burgundy,

na-egosi nsonaazụ nyocha nke ọma C. pneumoniae IgG.Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ewu mgbochi òké IgG/mouse IgGgold conjugate n'agbanyeghị ọnụnọ T band nwere agba.Ma ọ bụghị ya, nsonaazụ ule

adịghị mma yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.

Strip B: Mgbe etinyere oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette ule, ihe nlere ahụ na-akwaga site na capillary edinam gafee cassette.C.pneumoniae IgM mgbochi ma ọ bụrụ na ọ dị na ihe nlere anya ga-ejikọta na C. pneumoniae Antigen conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ site na mgbochi mgbochi mmadụ IgM nke Mouse kpuchie ya, na-akpụ otu agba T nwere agba burgundy,

na-egosi nsonaazụ nyocha nke ọma C. pneumoniae IgM.Enweghị T-band na-egosi nsonaazụ na-adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosipụta eriri agba agba burgundy nke immunocomplex nke ewu mgbochi òké IgG/mouse IgGgold conjugate n'agbanyeghị ọnụnọ T band nwere agba.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.